Fuscibacter oryzae gen. nov., sp. nov., a phosphate-solubilizing bacterium isolated from the rhizosphere of rice plant

An ovoid to rod shaped, white brown pigmented, facultative anaerobic, mesophilic, non-phototrophic, Gram-staining-negative, non-motile, multiply by binary fission designated strain KVB23T, which was isolated from root of rice plant, near Ilsan, South Korea, investigated for its taxonomic position polyphasic approach. Optimal growth found occur at 30˚C, pH 6.5 and in the absence NaCl on R2A. Phylogenetic analysis based 16S rRNA gene sequence KVB23T revealed that it formed a distinct lineage, as separate deep branch within family Rhodobacteriaceae, with < 96.5% similarity representatives genera Rhodobacter, Xinfangfangia, Tabrizicola, Falsirhodobacter, Haematobacter, Paenirhodobacter, Pseudorhodobacter Pararhodobacter. Based sequences most closely related Tabrizicola fusiformis KCTC 62105 T (96.5%) Rhodobacter thermarum 52712 (96.2%). The draft genome 3.80 bp long DNA G + C content 63.1%. Genome harboured clusters tryptophan cobalamin biosynthesis. contained Q-10 sole respiratory quinone. predominant fatty acids were consist C16:0, C18:0 summed feature 8 (comprising C18:1 ω7c / or ω6). polar lipids identified diphosphatidylglycerol, phosphatidylethanolamine, seven unidentified phosphoglycolipids, two aminophosphoglycolipid, one glycolipid four lipids. Phosphate-solubilizing bacteria have ability dissolve insoluble phosphates enhance soil fertility. Strain can solubilize calcium phosphate tribasic. Phosphate solubilizing biosynthesis property could be possible factor increase plant. Differential phenotypic, chemotaxonomic genotypic properties, together phylogenetic distinctiveness, demonstrated represent novel genus Rhodobacteriaceae family, name Fuscibacter oryzae gen. nov., sp. nov. is proposed, type KVB23T(= KACC 21711 = NBRC 114716 T).